Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 7 de 7
Filter
1.
Chinese Journal of Immunology ; (12): 296-300, 2018.
Article in Chinese | WPRIM | ID: wpr-702721

ABSTRACT

The microparticle is a kind of small particle formed in the process of cell activation and apoptosis.Currently,the mi-croparticle is taken as the main medium of inflammatory reaction.However,it may also play a role of atherosclerotic plaque formation and subsequent onset,development and formation of acute coronary syndrome.Furthermore,the microparticle participates in the atherosclerotic plaque formation and rupture,coagulation startup and thrombus formation.Through the study of microparticle,the coronary atherosclerotic heart disease pathogenesis can be deeply understood.

2.
Chinese Journal of Pathology ; (12): 745-748, 2011.
Article in Chinese | WPRIM | ID: wpr-358247

ABSTRACT

<p><b>OBJECTIVE</b>To study immunohistochemical expression of GADD153 and assess its usefulness as markers in the differential diagnoses in follicular tumors of the thyroid.</p><p><b>METHODS</b>Immunohistochemical staining was performed on formalin-fixed paraffin-embedded tissue of 34 cases of follicular thyroid adenomas (FTA), 46 cases of follicular thyroid carcinomas (FTC), 29 cases of follicular variant papillary carcinomas (FVPC).</p><p><b>RESULTS</b>(1) GADD153 was expressed in cell nucleus with positive or strong positive expression in FTC, and no or weak expression in FTA and FVPC. The positive expressions of GADD153 were present in 38 of 46(82.6%) in FTC, 11 of 34(32.4%) in FTA and three of 29(10.3%) in FVPC, the positive expression rate in FTC was obviously higher than that in FTA and in FVPC, the differences were statistically significant (χ² = 20.80 and 37.48; P < 0.01). (2) CK19, Galectin-3 (Gal-3) and HBME-1 were all expressed in the cytoplasm, the positive expressions of CK19, Gal-3 and HBME-1 were present in 54.3% (25/46), 67.4% (31/46) and 58.7% (27/46) in FTC; 50.0% (17/34), 29.4% (10/34) and 32.4% (11/34) in FTA; 100% (29/29), 93.1% (27/29) and 89.7% (26/29) in FVPC, the differences were statistically significant as well (χ² = 21.20 and 8.22; P < 0.01). (3) According to the expressions of CK19, Gal-3, HBME-1 and GADD153, we divided the results into low expression group (0 or 1+) and high expression group (2+ or 3+), the sensitivity and the specificity were calculated. in FTA, the sensitivity were 26.5%, 8.8%, 2.9% and 11.8%; the specificity were 50.7%, 52.0%, 54.7% and 58.7%. in FTC, the sensitivity were 19.6%, 26.1%, 23.9% and 65.2%; the specificity were 41.3%, 57.1%, 62.0% and 92.1%. in FVPC, the sensitivity were 96.6%, 82.8%, 79.3% and 3.4%; the specificity were 77.5%, 81.3%, 85.0% and 57.5%.</p><p><b>CONCLUSIONS</b>The sensitivity and the specificity of GADD153 expression are well for diagnosing FTC, and CK19, Gal-3, HBME-1 are well for FVPC. The four markers when used in combination, are better to identify the follicular tumors of the thyroid.</p>


Subject(s)
Humans , Adenocarcinoma, Follicular , Diagnosis , Metabolism , Pathology , Adenoma , Diagnosis , Metabolism , Pathology , Biomarkers, Tumor , Metabolism , Carcinoma, Papillary, Follicular , Diagnosis , Metabolism , Pathology , Diagnosis, Differential , Galectin 3 , Metabolism , Keratin-19 , Metabolism , Sensitivity and Specificity , Thyroid Neoplasms , Diagnosis , Metabolism , Pathology , Transcription Factor CHOP , Metabolism
3.
Chinese Journal of Pathology ; (12): 671-674, 2010.
Article in Chinese | WPRIM | ID: wpr-295156

ABSTRACT

<p><b>OBJECTIVE</b>To study the clinicopathologic features of uterine papillary serous carcinoma (UPSC) and the roles of adjuvant therapy.</p><p><b>METHODS</b>Sixty-one cases of UPSC with operation done and followed up for a period of 4 to 9 years were enrolled into the study. The histology of slides specimens were reviewed and immunohistochemical study was performed. The follow-up and survival data were analyzed.</p><p><b>RESULTS</b>All of the 61 patients were post-menopausal, with a median age of 68 years. The clinical presentations included abnormal vaginal bleeding, abdominal symptoms and abnormal Pap smears. The median size of the tumors was 7.5 cm (range=1.2 to 14.8 cm). There were 27.9% cases in FIGO stage I (8.2% in stage IA, 14.8% in stage IB and 4.9% in stage IC), 9.8% in stage II, 32.8% in stage III and 29.5% in FIGO stage IV. The histologic features were similar to those of the ovarian counterpart, with tumor cells containing the high-grade nuclei and arranged in complex papillae. Psammoma bodies were identified in 24.6% of the cases. Immunohistochemical study showed that the tumor cells demonstrated diffuse and strong nuclear staining for p53 and Ki-67. They were negative for estrogen receptor and progesterone receptor. Fifteen of the 61 cases (24.6%) showed no evidence of myometrial invasion. However, ten of the 15 cases had extrauterine disease, with peritoneal (6/15) and nodal (9/15) involvement. Tumors with deep myometrial invasion, lymphovascular permeation and nodal metastasis were associated with worse prognosis by univariate analysis. Fifty-six patients received adjuvant therapy. The number of patients receiving adjuvant chemotherapy alone, adjuvant radiotherapy alone and combined adjuvant chemotherapy/radiotherapy were 42, 24 and 10, respectively. The median survivals of the chemotherapy group and non-chemotherapy group (with or without radiotherapy) were 66.4 months and 32.8 months, respectively.</p><p><b>CONCLUSIONS</b>UPSC has distinctive clinical and pathologic features. The tumor stage, lymph node status, lymphovascular permeation and depth of myometrial invasion were important prognostic factors. Adjuvant chemotherapy for stage III/IV tumors or recurrent UPSC may have survival benefit.</p>


Subject(s)
Aged , Aged, 80 and over , Female , Humans , Middle Aged , Antineoplastic Combined Chemotherapy Protocols , Therapeutic Uses , Carcinoma, Papillary , Drug Therapy , Pathology , Radiotherapy , General Surgery , Chemotherapy, Adjuvant , Cisplatin , Cystadenocarcinoma, Serous , Drug Therapy , Pathology , Radiotherapy , General Surgery , Follow-Up Studies , Lymphatic Metastasis , Menopause , Neoplasm Invasiveness , Neoplasm Staging , Paclitaxel , Radiotherapy, Adjuvant , Survival Rate , Uterine Neoplasms , Drug Therapy , Pathology , Radiotherapy , General Surgery
4.
Chinese Journal of Pathology ; (12): 617-620, 2009.
Article in Chinese | WPRIM | ID: wpr-273487

ABSTRACT

<p><b>OBJECTIVE</b>To study the clinicopathologic features of gastrointestinal stromal tumor (GIST) of small intestine with lymph node metastasis and evaluate the respond to imatinib mesylate (Glivec) therapy.</p><p><b>METHODS</b>Two cases of GIST of small intestine associated with lymph node metastasis were collected and investigated by light microscopy and immunohistochemistry. Mutation in exon 9, 11 and of c-kit gene were analyzed by polymerase chain reaction and DNA sequencing.</p><p><b>RESULTS</b>The cases presented as small intestinal mass of irregular shape. Histologically, the tumors consisted of epithelioid and spindled cells, with areas of coagulative necrosis and hemorrhage. The mitotic count measured about 2 per 50 high-power fields. Immunohistochemical study showed that the tumor cells were diffusely distributed and strongly positive for CD117. Mutation analysis revealed that case 1 had an in-frame deletion of 11 amino-acid residues corresponding to 559-569 and carried two missense mutations involving codons 570, 571 in exon 11 of c-kit gene. Case 2 revealed an in-frame deletion involved condons 559-565 in exon 11 of c-kit gene. These two cases were all underwent primary chemotherapy with imatinib mesylate and without new tumor was found during follow-up periods (18, 26 months) after operation.</p><p><b>CONCLUSIONS</b>GIST with nodal metastasis is very rare and needs to be distinguished from other soft tissue sarcomas occurring in this site. The responsiveness to imatinib mesylate therapy correlates with the mutation status of c-kit gene.</p>


Subject(s)
Adult , Humans , Male , Middle Aged , Antineoplastic Agents , Therapeutic Uses , Benzamides , Codon , Exons , Follow-Up Studies , Gastrointestinal Stromal Tumors , Drug Therapy , Metabolism , Pathology , General Surgery , Imatinib Mesylate , Lymphatic Metastasis , Mutation , Piperazines , Therapeutic Uses , Proto-Oncogene Proteins c-kit , Genetics , Metabolism , Pyrimidines , Therapeutic Uses
5.
Chinese Journal of Pathology ; (12): 118-121, 2007.
Article in Chinese | WPRIM | ID: wpr-333956

ABSTRACT

<p><b>OBJECTIVE</b>To study the clinicopathologic features of sclerosing angiomatoid nodular transformation of spleen and its differential diagnosis.</p><p><b>METHODS</b>The clinicopathologic characteristics and immunophenotype of 4 cases of sclerosing angiomatoid nodular transformation of spleen were studied.</p><p><b>RESULTS</b>Histologically, all cases were characterized by multiple angiomatoid nodules of various sizes in a fibrosclerotic stroma. The nodules were round and sometimes convoluted. They were composed of slit-like, irregular-shaped or slightly dilated vascular spaces lined by plump endothelial cells and interspersed with a population of spindly or ovoid cells. Immunohistochemical study showed a heterogeneous staining pattern, with the lining cells of the small capillaries expressing CD34 and those of the sinusoid-like structures expressing CD8. CD31 highlighted both the lining cells and interspersed cells, resulting in a complex meshwork. The lining cells were also focally positive for CD68. Smooth muscle actin revealed conglomerates of spindly shaped cells around and between the vascular channels. These spindly shaped cells in the intervening stroma were focally positive for actin, but negative for desmin, CD21 and CD35.</p><p><b>CONCLUSIONS</b>Sclerosing angiomatoid nodular transformation is a rarely encountered benign lesion of the spleen, which should be distinguished from other angiomatoid tumors and tumor-like lesions.</p>


Subject(s)
Adult , Female , Humans , Male , Middle Aged , Angiomatosis , Metabolism , Pathology , General Surgery , Antigens, CD34 , Metabolism , CD8 Antigens , Metabolism , Diagnosis, Differential , Follow-Up Studies , Hamartoma , Pathology , Hemangioma , Pathology , Immunohistochemistry , Platelet Endothelial Cell Adhesion Molecule-1 , Metabolism , Sclerosis , Pathology , Spleen , Pathology , Splenectomy , Splenic Diseases , Metabolism , Pathology , General Surgery , Splenic Neoplasms , Pathology
6.
Chinese Journal of Pathology ; (12): 605-608, 2007.
Article in Chinese | WPRIM | ID: wpr-347719

ABSTRACT

<p><b>OBJECTIVE</b>To study the clinicopathologic features, diagnosis and differential diagnosis of inflammatory myofibroblastic tumor of the urinary bladder.</p><p><b>METHODS</b>Excisional specimens from 5 cases of vesical inflammatory myofibroblastic tumor were studied by light microscopy and immunohistochemistry (EnVision). The clinical data were also analyzed.</p><p><b>RESULTS</b>Among the 5 patients studied, 3 were males and 2 were females. The age of the patients ranged from 10 to 53 years (mean age = 35 years). The most common clinical presentation was micturition pain and hematuria. Three cases were located at the dome of the urinary bladder and the remaining 2 cases were found in the left lateral wall. Histologically, the tumor varied from myxoid to highly cellular. The tumor cells were spindle to stellate in shape, widely separated or showed a compact fascicular pattern. There were often associated with mixed inflammatory infiltrates and an irregular meshwork of small dilated vessels. Immunohistochemical study showed that the tumor cells expressed AE1/AE3 (5/5), vimentin (5/5), smooth muscle actin (5/5), calponin (5/5), caldesmon (3/5), desmin (4/5) and anaplastic lymphoma kinase protein (4/5). Follow-up data were available in 4 patients and none had local recurrence or died of this disease.</p><p><b>CONCLUSION</b>Inflammatory myofibroblastic tumour of urinary bladder is a rarely encountered but distinctive neoplasm with intermediate malignant potential.</p>


Subject(s)
Adolescent , Child , Female , Humans , Male , Middle Aged , Actins , Metabolism , Anion Exchange Protein 1, Erythrocyte , Metabolism , Calcium-Binding Proteins , Metabolism , Cystectomy , Methods , Diagnosis, Differential , Fibrosarcoma , Pathology , Follow-Up Studies , Inflammation , Pathology , Leiomyosarcoma , Pathology , Microfilament Proteins , Metabolism , Neoplasms, Muscle Tissue , Metabolism , Pathology , General Surgery , Protein-Tyrosine Kinases , Metabolism , Receptor Protein-Tyrosine Kinases , Rhabdomyosarcoma , Pathology , Survival Rate , Urinary Bladder Neoplasms , Metabolism , Pathology , General Surgery , Vimentin , Metabolism
7.
Chinese Journal of Pathology ; (12): 212-216, 2004.
Article in Chinese | WPRIM | ID: wpr-283545

ABSTRACT

<p><b>OBJECTIVE</b>To study immunohistochemical expression of cytokeratin19 (CK19), galectin-3 (Gal-3) and HBME-1 in thyroid lesions and to assess their usefulness as markers in the differential diagnoses of thyroid nodular lesions.</p><p><b>METHODS</b>Immunohistochemical staining was performed on formalin-fixed paraffin-embedded tissue of 21 cases of nodular goiters, 14 cases of toxic goiters, 15 cases of follicular adenomas (FA), 13 cases of follicular carcinomas (FC), 13 cases of follicular variant papillary carcinomas (FVPC) and 48 cases of classic papillary carcinomas (CPC).</p><p><b>RESULTS</b>All three markers were expressed in the cytoplasm with no or weak expression in benign lesions and diffuse and strong in malignant cases. Positive expressions of CK19, Gal-3 and HBME-1 were present in 11of 21, two of 21, four of 21 in nodular goiters, seven of 14, one of 14, one of 14 in toxic goiters, nine of 15, two of 15, two of 15 in FA, 10 of 13, eight of 13, seven of 13 in FC, 13 of 13, 11 of 13, 12 of 13 in FVPC, and 48 of 48, 45 of 48, 46 of 48 in CPC. The expression rates of the three markers between benign lesions (nodular goiters, toxic goiters and FA) and malignant lesions (FA, FVPC and CPC) were statistically significant. Among the three follicular lesions (FA, FC and FVPC), the differences were statistically significant as well. Nine, seven and six cases were negative for all three markers in nodular goiters, toxic goiters and FA, respectively. Only one case in FC was negative for all three markers, no case was all negative in FVPC and CPC; the rate of one case with two or more positive marker expression in nodular goiters, toxic goiters, FA, FC, FVPC and PC was 14.2% (3/21), 21.43% (3/14), 20.0% (3/15), 69.2% (9/13), 92.3% (12/13), 100.0% (48/48), the differences between benign lesions and malignant lesions and between FA, FC and FVPC were also statistically significant.</p><p><b>CONCLUSIONS</b>Immunohistochemical stains of CK19, Gal-3 and HBME-1, especially when used in combination, can be an important adjunct to the histopathological diagnoses of thyroid lesions.</p>


Subject(s)
Humans , Adenocarcinoma, Follicular , Chemistry , Diagnosis , Pathology , Adenoma , Chemistry , Diagnosis , Pathology , Biomarkers, Tumor , Genetics , Carcinoma, Papillary, Follicular , Pathology , Diagnosis, Differential , Galectin 3 , Genetics , Goiter, Nodular , Metabolism , Pathology , Immunohistochemistry , Keratins , Genetics , Thyroid Neoplasms , Chemistry , Diagnosis , Pathology , Thyroid Nodule , Chemistry , Diagnosis , Pathology
SELECTION OF CITATIONS
SEARCH DETAIL